Jung P.T., Briegel-Williams L., Werner L., Jost E., Schultz M., Nuernberg D.J., Grube M. & Lakatos M.
(2024):
A direct PCR approach with low-biomass insert opens new horizons for molecular sciences on cryptogam communities -
Applied and Environmental Microbiology,
2024: 1-16
Molecular sequence data have transformed research on cryptogams (e.g., lichens, microalgae, fungi, and symbionts thereof) but methods are still strongly hampered by the small size and intermingled growth of the target organisms, poor cultivability and detrimental effects of their secondary metabolites. Here, we aim to showcase examples on which a modified direct PCR approach for diverse aspects of molecular work on environmental samples concerning biocrusts, biofilms, and cryptogams gives new options …
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