We present a list of 189 lichenised and two non- or semi-lichenised fungi recorded in western Bohemia in April 2023 during the 28th Spring Meeting of the Bryological and Lichenological Section of the Czech Botanical Society. We focused on various habitats including a uranium mine heap, old limestone quarries, a quaternary volcano, a quarzite ridge as well as old trees in allees, and a chateau park. Several rare and remarkable species were found in the region, for example Acarospora versicolor, Calicium viride, Caloplaca limonia, Cetraria sepincola, Chaenotheca phaeocephala, Cladonia cariosa, Melanohalea exasperata, Micarea deminuta, M. nigella, Parmotrema perlatum, Ramalina fraxinea, Rhizocarpon eupetraeum, Sarcogyne hypophaeoides, Sclerophora peronella, and Stereocaulon condensatum. Keywords: biodiversity, rare species.