The department of Aude (Occitanie region, France) is one of the most biodiverse in mainland France, yet its land remains largely unexplored, leaving numerous lichen-forming fungi species undocumented. Herein, we document the discovery of 40 previously unrecorded species within the Aude department: Coniocarpon cinnabarinum, Alyxoria variiformis, Pseudoschismatomma rufescens, Acarospora glaucocarpa, Cladonia cervicornis, C. humilis, C. portentosa, C. squamosa var. subsquamosa, C. subulata, Squamarina gypsacea, S. periculosa, Stereocaulon evolutum, Parmelia omphalodes, Pleurosticta acetabulum, Usnea cornuta, U. esperantiana, U. flammea, U. glabrescens, U. rubicunda, Xanthoparmelia verruculifera, Psora testacea, Ramalina fraxinea, Thalloidima opuntioides, Sphaerophorus globosus, Romjularia lurida, Leprocaulon quisquiliare, Diploschistes scruposus, Graphis betulina, G. elegans, Scytinium lichenoides, Fuscopannaria ignobilis, Nephroma tangeriense, Peltigera canina, Peltigera hymenina, Rhizocarpon lecanorinum, Calogaya decipiens subsp. decipiens, Kuettlingeria teicholyta, Polycauliona polycarpa, Umbilicaria polyphylla, Rhytidhysteron hysterinum. Additionally, we report the first occurrence of Usnea fulvoreagens in the Lot department (Occitanie region, France). These findings contribute to our understanding of the remarkable lichen diversity within the Aude department, emphasizing the need for further exploration and conservation efforts in this region. Key words: Lichen survey, ZNIEFF, new records.