A new species in the genus Acarospora (Acarosporaceae, lichenized ascomycetes), from Darel Valley, Gilgit Baltistan, Pakistan, is described and illustrated here under the name Acarospora pakistanica. It is characterized by thicker epihymenium 30-60 mu m, taller hymenium 100-150 mu m, and larger ascospores 5-7 x 1-3 mu m. A detailed description of this taxon is provided. evolutionary relationships, taxonomy, novel species, geographic distribution