Use of fungal mycelium as biosupport in the formation of lichen-like structure: recovery of algal grown in sugarcane molasses for lipid accumulation and balanced fatty acid profile
- Author:
- Zorn S., Carvalho A., Bento H., Gambarato B., Pedro G., da Silva A., Gonçalves R., Da Rós P. & Silva M.
- Year:
- 2022
- Journal:
- Membranes
- Pages:
- 12(3): 258 [15 p.]
- Url:
- https://doi.org/10.3390/membranes12030258
In this study, a lichen-like structure was obtained through the production of a unique biomass, formed by algae cells of Scenedesmus obliquus adhering to the mycelium of filamentous fungal Mucor circinelloides. This structure was composed in two steps; in the first one, microalgal cells and spores were incubated separately, and in the second one, after 72 h of growth, isolated, mature mycelium was harvested and added to cell culture. For spores’ incubation, a culture medium containing only 2 g·L−1 of glucose and minerals was used. This culture medium, with low sugar content, provided a fungal biomass to the anchorage of microalgae cells. WC medium was used without and with sugarcane molasses supplementation for microalgae cells’ incubation. The lichen-type structure that was formed resulted in 99.7% efficiency in the recovery of microalgae cells and in up to 80% efficiency in the recovery of algae biomass in the lichen biomass composition. In addition, the resulting consortium attained a satisfactory lipid accumulation value (38.2 wt%) with a balanced fatty acid composition of 52.7% saturated plus monounsaturated fatty acids and 47.4% polyunsaturated fatty acids. Since fungal species are easy to recover, unlike microalgae, the lichen-like structure produced indicates an efficient low-cost bioremediation and harvesting alternative; in addition, it provides an oleaginous biomass for various industrial applications.
Keywords: lichen-like structure; Scenedesmus obliquus; Mucor circinelloides; mature mycelium; fatty acid composition.
- Id:
- 34177
- Submitter:
- zdenek
- Post_time:
- Friday, 25 February 2022 09:36