Insights into in vitro phenotypic plasticity, growth and secondary metabolites of the mycobiont isolated from the lichen Platygramme caesiopruinosa
- Author:
- Shanmugam K., Srinivasan M. & Neelakantan H.G.
- Year:
- 2022
- Journal:
- Archives of Microbiology
- Pages:
- 204: 90 [16 p.]
- Url:
- https://doi.org/10.1007/s00203-021-02685-w
The choice of inoculum for successful isolation and establishment of axenic lichen mycobiont culture is a key step towards
eliminating endolichenic and lichenicolous fungi and other microbial contamination. The nutritional requirements of each
lichen species are unique. This work reports on the isolation, phenotypic plasticity, growth and secondary metabolites from
mycobiont culture of the pantropical lichen Platygramme caesiopruinosa. Media composition [Malt yeast extract (MY),
Modified Murashige and Skoog (MMS) and Lilly and Barnett (LB) media] was optimized to determine nutritional requirements
for optimal growth of this species as assessed by dry biomass and the occurrence of secondary metabolite. Furthermore,
the role of different carbon sources in affecting growth, growth stages, phenotypic plasticity, biomass and spectrum
of secondary metabolites produced of this mycobiont was examined. The molecular identity of the mycobiont culture was
confirmed by amplifying mitochondrial small subunit (mtSSU) sequences. Cultures showed optimum biomass production
in MY medium with 10% sucrose. The secondary metabolite profiles for each culture treatment were characterized using
High-performance Thin-Layer Chromatography (HPTLC) and Gas Chromatography with Mass Spectrometric (GC–MS)
analysis. The HPTLC spectral comparison, phenolic and iodine confirmatory analysis revealed the absence of phenolic
metabolites and the presence of non-phenolic metabolites in mycobiont extracts, while GC–MS analysis revealed the biosynthesis
of side chain fatty acids, hydrocarbons and sugar alcohol in mycobiont cultures treated with 10% supplemented
sucrose as a carbon source.
Keywords: Mycobiont culture · Phenotypic plasticity ·
Secondary metabolites · Mitochondrial small subunit.
- Id:
- 34024
- Submitter:
- zdenek
- Post_time:
- Monday, 03 January 2022 11:02