[in Japanese with English abstract: ] Lichen flora of the Nagai Park (Osaka City) was investigated. As a result, 16 species of 13 genera of 8 families in Dothideomycetes and Lecanoromycetes were recognized. Lepraria ecorticata (J.R.Laundon) Kukwa and L. leuckertiana (Zedda) L.Saag were new to Japan, and Amandinea punctata (Hoffm.) Coppins & Scheid. and Punctelia borreri (Sm.) Krog were the first record in Osaka Prefecture. Key Words: urban biodiversity; cryptogamic flora; Amandinea; Lepraria; Punctelia.