94 species of lichens and lichenicolous fungi (43 genera, 22 families, 11 orders) are found inBurguns’ka balka raivne in southern Ukraine (Khersons`ka oblast / region). Acrocordia subglobosa (Vězda) Vězda & Poelt, Arthonia lecanorina (Almq.) R. Sant., Athelia arachnoidea(With.) J. R. Laundon, Collema fuscoviridis (With.) J. R. Laundon, Polycoccum marmoratum (Krempelh.) D. Hawksw. are revealed to be new to the steppe zone of Ukraine. 56 species (58,4%)are saxicolous occurring on limestone outcrops, 17 species (17,7%) are corticolous and and 17species (17,7%) are lichenicolous. Three species (3,1%) are considered ubiquists with occurring onvariety of substrats. The annotated list of taxa is compiled Key words: biodiversity, calcicolous lichens, southern Ukraine, steppe zone. [In Ukrainian with English abstract]