Gasulla F., Guéra A. & Barreno E.
“A simple and rapid method for isolating lichen photobionts”
For decades, lichenologists have developed numerous
and varied methods to isolate lichen photobionts.
Most procedures are tedious, slow, and require several
months after the initial isolation to obtain clones. Furthermore,
the purity of the isolated photobionts obtained by
more rapid methods is not sufficient to establish phycobiont
axenic cultures. We have developed a new method for
isolating lichen photobionts from fruticose, foliose and
crustose lichens. Basically, it involves homogenization …