During our research on Acarosporaceae species, we found three new pruinose species, Sarcogyne bayingolinensis, S. parasitica, and S. yiliensis, and one new species to China, Acarospora iqbalii, based on a combination of morphological, chemical, and phylogenetic analysis. All three new species have IKI+ blue hymenial gel. Sarcogyne bayingolinensis is characterized by lobate thallus margin and no pruina surrounding the apothecial disc. Sarcogyne parasitica is characterized by areoles with deep fissures surrounding the apothecial disc and exhibits a parasitic lifestyle. Sarcogyne yiliensis is characterized by thin hymenium (50–60 μm), and tall subhymenium (50–80 μm). In addition, we discovered that A. tianshanica exhibits two chemical types (with or without gyrophoric acid), and we supplemented the molecular data of A. glaucocarpa from China. A key for the Acarosporaceae species with pruina reported in China is also provided. Key words: Lichenized fungi, new record, new species, phylogeny, taxonomy.