The aim of the present study is to isolate and analyse the photobionts from lichens in different habitats of Northern Odisha. A total of 24 species of broadly distributed lichen genera i.e., Parmotrema A. Massal., Lecanora Ach., Coccocarpia Pers., Graphis Adans., Phaeographis Müll.Arg., Trypethelium Spreng., Parmelia Ach., Dirinaria (Tuck.) Clem., Buellia Alyssum L., Heterodermia Trevis, Hyperphyscia Müll. Arg., Letrouitia Hafellner & Bellem., Malmidia Kalb, Xanthoria (Fr.) Th.Fr. from different families spread across the floristic zones of the northern Odisha sector, were selected for photobiont isolation. Trebouxia Pyum, Trentepohlia Mart., Nostoc Vaucher ex Bornet et Flahault, Chroococcus Nägeli, and Scytonema C. Agardh ex Bornet & Flahault are found in lichens as a photobiont. All photobionts were further identified based on morphological and molecular approaches, including 18S rRNA, 16S rRNA, and ITS sequences which showed a wide differentiation of species diversity in Trebouxia, Trentepohlia and Chroococcus. Further to this study, all photobiont cultures were preserved for future biotechnological prospection. Keywords: diversity, isolation, lichen, Northern Odisha, India, photobiont.