Two new lichenized species, Chicitaea yueliangshanensis and Coniocarpon chishuiense are described through the combination of morphological characteristics, chemical profiling and phylogenetic analyses, along with a new geographical record of Synarthonia inconspicua, collected from Guizhou, China. Chicitaea yueliangshanensis is characterized by prominent lecanorine apothecia with a black, epruinose, ± flat disc and lacking isidia and soredia. This species contains perlatolic acid, 2’-O-methylperlatolic acid and an unidentified lichen substance. It forms a well-supported singleton based on both ML and Bayesian analyses in the phylogenetic tree. Another new species, Coniocarpon chishuiense is distinguished by irregularly rounded to elliptical ascomata with epruinose disc and the presence of psoromic acid along with an unknown compound. Detailed morphological descriptions, illustrations and a compiled species checklist summarizing diagnostic features are provided. Key words: 2 new species, geographical record, lichenized fungi, morphology, phylogeny, taxonomy.