We present here interesting records of 69 lichens (incl. one semilichen) and 13 lichenicolous fungi from Western Bohemia (Pilsen and Karlovy Vary Regions). In total, 42 recorded taxa belong to important categories of the Red list of lichens of the Czech Republic according to DaLiBor for 2023: C1 (critically endangered species – 6 taxa), C2 (strongly endangered species – 8) and C3 (endangered species – 30). Four taxa (Arthonia coronata, Porpidia flavocruenta, Thelenella modesta and Zyzygomyces aipoliae) are new for the Czech Republic. Lecidea albofuscescens, until recently considered extinct in the Czech Republic, was recorded after more than 60 years at its second locality in the country. Keywords: biodiversity, expanding species, first records for country, lichen-forming fungi, rare species.