In this study, taxonomic investigations were conducted on six lichenized fungi species from Robert Island (Antarctic Peninsula). The anatomical and morphological characteristics of the specimens were examined, along with molecular data. Internal Transcribed Spacer (ITS) primers were used as molecular markers for six species. Phylogenetic analyses were performed using sequences obtained from both the collected specimens and reference data from GenBank. The phylogenetic relationships and evolutionary tree construction were based on the Maximum Likelihood method. As a result of the study, the following lichenized fungi species were identified: Austrolecia antarctica Hertel, Lecanora polytropa (Ehrh.) Rabenh., Placopsis antarctica (Nyl.) I.M.Lamb, Rhizocarpon geminatum Körb., Rhizocarpon polycarpum (Hepp) Th.Fr., Usnea aurantiacoatra (Jacq) Bory. Keywords: Antarctic lichens, ITS Region, Lichenized fungi, Robert Island, Phylogenetic analysis.