Hyphomycetes is an artificial group of asexual fungi with an estimated 2200 recognizable genera. These fungi have crucial ecological and biotechnological significance by decomposing organic matter, facilitating nutrient recycling, and providing valuable metabolites, enzymes, and proteins for various applications in medicine, industry, and agriculture. Specifically, hyaline-spored hyphomycetes refer to hyphomycetes that produce colorless (hyaline) conidia. In this study, a comprehensive outline for hyaline-spored hyphomycetes is provided and includes 1237 genera with 151 synonyms, which are distributed among six phyla, 27 classes, 97 orders, and 239 families. At the phylum level, Ascomycota (1157 genera) is the dominant group of hyaline-spored hyphomycetes, with Sordariomycetes (506 genera) as the dominant class and Hypocreales (216 genera) as the dominant order in Ascomycota. In Basidiomycota, Agaricomycetes (46 genera) is the dominant class and Agaricales (11 genera) stands as the dominant order. For each accepted genus, notes including sexual morphs, synasexual morph, DNA sequence data and morphology are provided. Based on both morphology and phylogeny, the taxonomic position for 38 genera is re-organized, 22 of which were previously located in Ascomycota genera incertae sedis. DNA sequence data is one of the key components for each genus in our notes. This study represents the most comprehensive analysis to date of hyaline-spored hyphomycetes subjected to multi-gene phylogenetic analysis with combined LSU, SSU and rpb2 DNA sequence data. This analysis encompasses 754 hyaline-spored hyphomycetous genera, and recognized three phyla clades, 25 classes and class-level clades, 107 orders and order-level clades, as well as 264 families and family-level clades. Thirty-eight taxa from 30 genera were documented based on fresh collections, utilizing both morphological characteristics and multi-gene phylogeny, resulting in one new genus Parapleurothecium, with one new combination P. obovoideum; 14 new species, viz. Aciculomyces hyalosporus, Beltraniella hyalospora, Cylindrotrichum hyalosporum, Haplographium hyalosporum, Mariannaea hyalina, Neohelicomyces astrictus, N. brunneus, Parasympodiella hyalospora, Pleurotheciella brevis, Pleurothecium hyalosporum, Pseudonectria hyalina, Rhamphoriopsis brevis, Sarocladium hyalosporum, Xylolentia oblongispora; and three new geographical records, viz. Monilochaetes regenerans, Subulispora longirostrata, Zygosporium pseudogibbum. Note: The authors missed in their outline the recently described monotypic lichenized genus Monilibrachium Vondrák et al. (2024, Preslia 96: 385).