The article presents the results of monitoring of rare and protected species of plants, fungi and lichens in the Aksai District of the Rostov Region. 26 species from the Red Data Book of the Rostov Region in 28 locations, including 5 species of Federal protection status are listed for the district. As a result of the work, information on the distribution in the region of 17 species of fungi and plants from the regional Red Data Book, in-cluding 6 species of Federal protection status, was supplemented and refined. 20 new locations of 13 species of fungi, mosses and vascular plants, including 11 locations of 6 species listed in the Red Data Book of the Russian Federation were revealed. Amanita vittadinii (Mоretti) Vittad., Polyporus rhizophilus Pat., Gymnostomum aeruginosum Sm., Hedysarum grandiflorum Pall. and Stipa zalesskii Wilensky were identified on the territory for the first time. The location of the moss Eucladium verticillatum (Brid.) B. S. G., which was known for the Aksai District from a single location, was confirmed. Another rare in the Rostov Region moss – Gymnostomum aeruginosum Sm. was recorded in this habitat for the first time for the Aksai District. 15 locations of 14 rare species of fungi, lichens and vascular plants were not confirmed. Keywords: rare species, monitoring, Aksaysky District, Red Data Book, Rostov Region.