Conference Paper [in Bulgarian with English summary :] The aim of this study is to present information on the di versi ty of lichenized fungi, established from Kupena Reserve. Field studies were conducted during the summer of 2014, following the method of linear transects. Processing of the collected specimens and applying of the spot test reactions with chemical reagents (C, K, KC, CK, Lugol's solution), and the tests of lichen thalli with UV light, were performed in laboratory. All ofthe photos were made with the aid of Canon PS digital cameras on binocular microscope Boeco BM-120 LM, and on Boeco B-3500 and Carl Zeiss binocular dissecting microscopes. As a result, 45 species are reported from Kupena Reserve. All of the species belong to Lecanoromycetes. Nearly half of them, 20 species (44%) are included in the Red List of lichenized fungi of Bulgaria. The lichen diversity from this protected area is presented mainly by lichens inhabiting rocks and soil, followed by species, registered on twigs and bark of trees or shrubs, and one species with leprose thallus found on moss. Future field studies, aimed at the establishment of endangered lichen species, and collecting of an additional information on the known diversity of the lichen mycota from this protected area, are needed. Key words: new data, old forests, Lecanoromycetes.