Forty species of lichens are reported from the nature reserve area as a result of field and laboratory work in 2014. Thirteen species, Amandinea punctata, Caloplaca subpallida s.l., Cladonia coniocraea, C. fimbriata, Evernia prunastri, Lecanora carpinea, Lecidea confluens, Lecidella elaeochroma, Opegrapha atra, Parmelina quercina, Pertusaria albescens, Rinodina confragosa, and Sarcogyne privigna, are reported for the first time from the valley of Struma River. Opegrapha atra is less known in Bulgaria. Brief notes and information on their distribution, locations and substrata are included. Key words: biodiversity, lichen mycota, protected area, Tisata.