Strategies to prevent (re-)growths on heritage surfaces are required to improve the management of biodeterioration issues. Nevertheless, (re-)colonization dynamics may be also limited by the divergence of current (micro-)climatic conditions from those of the past which allowed biomass accumulation, as modelized for the Mediterranean area. In this work, lichen communities were re-surveyed after eight years on the vertical walls of the House of the Ancient Hunt in Pompeii (VII,4,48) (S-Italy), displaying shifts in cover values varying for species and the cardinal aspect of walls. In particular, the hygrophytic Dirina massiliensis showed a regression on the S-facing wall, while the xerophytic Verrucaria macrostoma relatively increased and the mesophytic Lepraria lobificans was rather unchanged. To evaluate if these trends reflect a change in the wall microclimatic suitability, the photosynthetic activity of lichens (and of phototrophic biofilms as a comparison) was fluorimetrically monitored across the four seasons, under changing meteorological conditions, at different cardinal aspects and distances from the ground. GLM models indicated a maximum influence of spatial related factors (primary wall aspect) on the variability of basal fluorescence (F0) and maximum quantum yield (Fv/Fm), higher than that of lithobiont type and the temporal factors (month, interval from sunrise). Relationships between photosynthetic activity and meteorological conditions also showed aspect dependence, with rainfall and consequent wind-driven rain (WDR) having remarkable importance, particularly for S-aspect. On this aspect, the hygrophytic D. massiliensis showed prolonged photosynthetic inactivity, even under WDR favourable conditions, suggesting the loss of suitability of its current micro-niche and realizing expectations of climate change models. Keywords: Archaeological walls; Cardinal aspect; Fluorimetry; Mediterranean area; (Micro-)climatic factors; Preventive conservation; Wind-driven rain.