A method was developed to authenticate Tibet snow tea (Lichen Thamnolia spp.) by UV fluorescence and high resolution mass spectrometry with chemometrics analysis. The representative snow teas were collected from Shannan in Tibet Autonomous Region. Tibet snow teas can be discriminated by the high resolution mass spectrometry (HRMS) with statistical methods. Total flavonoids were founded including 4 mono-nuclear phenols, 3 depsides and 3 depsidones. There was a good correlation between the antioxidant activity and the total phenols and total flavonoids, as indicated by the correlation coefficient. Three biomarkers were semi-quantitatively analyzed by targeted metabolomics and presumed to interact with Alzheimer's disease through molecular docking, suggesting that they are the primary active components of Tibet snow tea in treating Alzheimer's disease. Keywords: Authenticity identification; Tibet snow tea; High resolution mass spectrometry; Chemometric analysis.