An attempt was made to study the antimicrobial activity of saxicolous lichen Roccella montagnei under invitro conditions. Theantimicrobial activities of Methanol, Ethanol, Ethyl Acetate and Acetone extracts of Roccella montagnei were assayed againstnine pathogenic microorganisms using the standard welldiffusion method. The Acetonic extract was found most effectiveantibacterial whereas the Ethanolic extract was found most effective antifungal against most of the organisms. The maximuminhibition zone was recorded in E. coli with inhibition zone 34 mm. fungal pathogens showed their inhibition zones in varyinglevels as 32 mm in Candida albicans and Fusarium oxysporium and 30 mm in Aspergillus niger. The present study reveals thatextracts obtained from R. montagnei have potential compounds that can lead to control of human pathogenic microorganismsin the future.