Dothideomycetes is the largest and most diverse fungal class of Ascomycota, comprising of about 20,000 species classified into 50 orders, 223 families and 1941 genera. Species in this class are characterised mainly by bitunicate asci with fissitunicate dehiscence and have different fungal lifestyles, including saprobic, pathogenic, endophytic, epiphytic, fungicolous, lichenicolous, lichenized and rock-inhabiting fungi. Since the last monograph of refined families of Dothideomycetes in 2020, numerous novel species, genera, families, and orders have been discovered. This has expanded information which has led to an increased knowledge in the classification of Dothideomycetes. In this paper, we provide a refined and updated documentation on 1941 generic types of Dothideomycetes as currently recognized. We accept ten orders with 30 families and 452 genera in Dothideomycetidae and four orders with 97 families and 739 genera in Pleosporomycetidae based on morphology and phylogeny. Thirty-six orders containing 59 families, and 390 genera are referred to as orders incertae sedis, and 40 families comprising 93 genera as families incertae sedis due to a lack of molecular or morphological evidence. We list ten taxa that are transferred to other classes and provide short notes based on recent publications. Reference specimens for Melaspileella proximella, Yoshinagaia quercus and Yoshinagella japonica and one epitype for Saccothecium sepincola are provided in this paper. Ascomycetes, biochemical importance, bitunicate, diversity, ecosystem, families, fissitunicate, GenBank, generic types, genus, herbarium, industrial relevance, morphology, orders, quarantine, species, subclass, taxonomy