A new compound, namely hopane-6α,16α,22-triol (1), along with 8 known compounds 2 to 9, leucotylin (2), 16β-acetoxy-hopane-6α,22-diol (3), 6α-acetoxyhopane-16β,22-diol (4), zeorin (5), 6α-acetoxyhopane-22-ol (6), ergosterol peroxide (7), brassicasterol (8), and atranorin (9), was isolated from the lichen Parmotrema sancti-angelii. The structures of all the isolated compounds 1 to 9 were fully characterized using spectroscopic data, as well as comparison with the previous literature data. Moreover, compounds 2 and 4 were assessed for their in vitro cytotoxicity against 3 human cancer cell lines.