Comparative analyses of antioxidant, cytotoxic, and anti-inflammatory activities of different Cladonia species and determination of fumarprotocetraric acid amounts

Author:
Kocakaya Z., Kocakaya M. & Karatoprak G.S.
Year:
2021
Journal:
Journal of Agriculture and Nature
Pages:
24(6): 1196-1207
Url:
https://doi.org/10.3390/ijms241713232
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In traditional medicine, Cladonia lichens are used for several disorders like hypertension, headaches, dizziness, cough, and menstrual disorders. The study aimed to determine the biological activities of 70% methanol extracts of nine Cladonia species from Turkey. The chemical composition was analyzed via spectrophotometric and chromatographic (HPLC) techniques. DPPH● (1,1-diphenyl-2-picrylhydrazyl) and ABTS+● (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities, inhibition of β-carotene/linoleic acid co-oxidation, and cytotoxicity on A549 and COLO205 cell lines were studied. Anti-inflammatory activity was assessed on the Raw 264.7 mouse macrophage cell line by determining nitric oxide (NO) levels. Among the studied Cladonia species Cladonia firma was found the most active extract in ABTS+● test system (2.58±0.07 mmol/L/Trolox) and also in the cytotoxicity and anti-inflammatory experiments. Cladonia pocillum, Cladonia rangiformis, and Cladonia foliaceae were also found to be active. Fumarprotocetraric acid amounts are between 1.89 -23.82 mg/gextract and fumarprotocetraric acid content did not show linearity with biological activities. It has been proved with this study that lichens traditionally used in treatment have as much biological activity as plants and can be evaluated in pharmaceuticals and cosmetics.
Id:
37612
Submitter:
jph
Post_time:
Friday, 31 January 2025 09:55