Galindez Island (65° 15′ S, 64° 15′ W) is one of the Argentine Islands located in the West Antarctic Peninsula, 5-6 km away from the main continent. It has a total surface area of 0.8 km2 and an annual temperature range of 9–13°С. There are not many studies providing information about lichenized fungi on Galindez Island. This study aimed to DNA barcode some lichenized and lichenicolous fungi from Galindez Island (Antarctic Peninsula, Antarctica). Lichenized fungi were collected from Galindez Island during the 2016-2017 Austral Summer. Stereo and light microscopes were used for the identification of lichen samples. After being anatomically and morphologically identified, DNA barcoding was carried out using the nrITS gene. As a result of the study, DNA barcoding of the following samples was successfully carried out: Lecanora intricata (Ach.) Ach., Mastodia tessellata (Hook. f. & Harv.) Hook. f. & Harv., Raesaenenia usneae (C.W. Dodge) Etayo & Pino-Bodas, Rhizocarpon grande (Flörke ex Flot.) Arnold, Umbilicaria umbilicarioides (Stein) Krog & Swinscow. Keywords: DNA barcoding, Antarctica, Antarctic Peninsula, lichenized fungi, lichen.