Lichenized fungi are the dominant elements of the Antarctic terrestrial vegetation. Recently, biodiversity studies of lichenized fungi in Antarctica have increased and the studies have started to become more phylogeny-based. Previous studies on the genera Amandinea, Buellia and Tetramelas in Antarctica and also Southern Hemisphere were mostly based on their anatomical-morphological and chemical properties. The present study is aimed at contributing to these three buellioid genera in Antarctica especially using molecular characters. As a result of studying Antarctic collections morphologically and phylogenetically based on nrITS and/or mtSSU gene data, we identified Amandinea babingtonii (Hook. f. & Taylor) Søchting & Øvstedal, Amandinea coniops (Wahlenb.) M. Choisy, Amandinea falklandica (Darb.) Elix & Kantvilas, Amandinea latemarginata (Darb.) Søchting & Øvstedal, Amandinea norconiops Elix, Amandinea petermannii (Hue) Matzer, H. Mayrhofer & Scheid, Buellia aethalea (Ach.) Th. Fr., Buellia frigida Darb., Buellia rodseppeltii Elix, Buelliarussa (Hue) Darb., Buellia subfrigida Mas. Inoue, Tetramelas anisomerus (Vain.) Elix, Tetramelas granulosus (Darb.) A. Nordin and Tetramelas pulverulentus (Anzi) A. Nordin & Tibell. Detailed descriptions, photographs and phylogenetic trees of the species are provided in this paper. Besides, nrITS and/or mtSSU nucleotide sequence data of B. subfrigida, B. rodseppeltii, A. babingtonii, A. falklandica, A. latemarginata, A. norconiops and T. granulosus species were obtained and uploaded to GenBank for the first time. Keywords: Antarctic lichens · Buellioid lichens · Biodiversity · Caliciaceae · DNA barcoding.