This document serves as the third supplement to earlier tabulations of species in the Hawaiian Islands, incorporating updates on native, non-indigenous, and fossil species. It highlights the unique biodiversity of Hawaii, shaped by geographic isolation and ecological diversity, with over 22,400 native species derived from approximately 1,000 original colonists. The report updates data on terrestrial and aquatic biota, emphasizes critical knowledge gaps, and introduces new records for fungi, plants, invertebrates, and vertebrates. It also details fossil records of coral, mollusks, and avian species. The findings underscore Hawaii's role as a biodiversity hotspot, critical for conservation and evolutionary studies