This supplement to earlier tabulations compiles updates on the biodiversity of Hawaii, reporting 22,056 species across various taxa, with 8,850 endemic and 4,465 non-indigenous species. The survey highlights significant refinements in species counts, particularly among protists, fungi, plants, and animals. It underscores critical gaps in understanding Hawaii's biota, with many undescribed species, and provides foundational data for conservation efforts and biological management.