Climatic conditions and physical features of the Eastern Himalayan region encompasses a rich diversity of lichen biota. However, the region’s lichens are still far from extensively explored. Here, we study the genus Pyrenula Ach. from the Dhubri district of Assam in North-East India. Twelve species of Pyrenula have been recorded, of which one of the species, P. chlorospila (Nyl.) Arnold, is a newly reported from India. Morphotaxonomic comments on this species, emphasizing its distribution, are provided. Keywords: New record, North-East India, taxonomy.