In recent decades, the occurrence of new diseases associated with pathogens in humans has become very common and has spurred efforts to exert control over various diseases. Lichen species such as Parmotrema austrosinense, P. hababianum, and P. tinctorum were collected from the Eastern Ghats of India, covering the Shevaroy hills in Tamil Nadu. The extracts were obtained by using various solvents; they were then subjected to antibacterial and various antioxidants assays, such as 2,2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and hydrogen peroxide. Benzene extract of P. tinctorum, showed an inhibition zone of 13.77 mm against Salmonella typhimurium, whereas in case of P. hababianum and P. austrosinense inhibition zones were 8.2 and 12.23 mm, respectively. Total antioxidant activity of acetone extract (IC50 18.41 μg/mL) of P. tinctorum was higher than that of other species. Methanol and benzene extracts of P. hababianum and P. austrosinense showed significant effects on free radical scavenging activity. Keywords: Lichen, Antibacterial, Antioxidant activity, DPPH assay, FRAP assay, IC50.