A sustainable application for the extraction of lichen metabolites from Usnea cornuta: nontargeted metabolomics and antioxidant activity
- Author:
- Castañeta G., Sepulveda B., Vargas R., Garcia-Beltran O., Simirgiotis M. & Areche C.
- Year:
- 2023
- Journal:
- Natural Product Research
- Pages:
- 37(12): 2076‒2082
- Url:
- https://doi.org/10.1080/14786419.2022.2116703
In this study, isolation and purification of lichen substances from
Usnea cornuta were performed using conventional solvents, green
solvents and green technologies. In addition, several lichen compounds were tentatively identified by UHPLC/ESI/MS/MS and
usnic acid, diffractaic and galbinic acids were quantified as well.
Limonene, ethyl lactate and methanol, were compared regarding
their extraction properties and antioxidant capacities, determined
by DPPH, ORAC, and FRAP assays. In the ethyl lactate, methanol
and limonene extracts, 28 compounds in all, were detected for
the first time by high resolution UHPLC-MS/MS fingerprinting.
Untargeted metabolomics tentatively identified 14 compounds
from the methanolic extract, 4 from limonene extract, and 20
metabolites from ethyl lactate extract. The green extract of ethyl
lactate showed a similar antioxidant capacity to toxic methanol
extract, except at ORAC assay where it was higher. Therefore,
ethyl lactate can replace methanol, to provide more sustainable
green chemistry methods.
Keywords: Alternative solvents; antioxidants; green chemistry; Usnea; LC/MS; lichens.
- Id:
- 35708
- Submitter:
- zpalice
- Post_time:
- Saturday, 05 August 2023 17:45