De novo mitochondrial genome sequencing of Cladonia subulata and phylogenetic analysis with other dissimilar species
- Author:
- Fang J., Mamut R., Wang L. & Anwar G.
- Year:
- 2023
- Journal:
- PLoS ONE
- Pages:
- 18(5): e0285818 [19 p.]
- Url:
- https://doi.org/10.1371/journal.pone.0285818
In this study, the complete mitochondrial genome of Cladonia subulata (L.) FH Wigg was
sequenced and assembled and then compared with those of other Cladonia species. The
mitogenome of Cladonia subulata, the type species of Cladonia, consisted of a circular DNA
molecule of 58,895 bp 44 genes (15 protein-coding genes, 2 rRNA genes, and 27 tRNA
genes). The base composition had shown an obvious AT preference, and all 27 tRNA genes
formed a typical clover structure. Comparison with other 7 Cladonia species indicated that
the duplication/loss of tRNAs had occurred during evolution, and introns appeared to explain
the variation in cox1 genes in Cladonia, the mitochondrial genome tends to be generally
conservative and local dynamic changes. Repeat sequences were mainly located in gene
intervals, which were mainly distributed among intergenic spacers and may cause rearrangement of the mitogenome. The phylogenetic results showed that Cladonia subulata and
C. polycarpoides were assigned to the Cladonia Subclade. The results add to the available
mitochondrial genome sequence information of Cladonia subulata, provide basic data for
the systematic development, resource protection, and genetic diversity research in Cladonia
subulata, and also provide theoretical support for further genomic research of lichens.
- Id:
- 35455
- Submitter:
- zpalice
- Post_time:
- Thursday, 25 May 2023 21:59