- Miral A., Jargeat P., Mambu L., Rouaud I., Tranchimand S. & Tomasi S.
- Environmental Microbiology Reports
- 14: 856–872
- DOI: 10.1111/1758-2229.13105
Recently, the study of the interactions within a microcosm between hosts
and their associated microbial communities drew an unprecedented interest
arising from the holobiont concept. Lichens, a symbiotic association
between a fungus and an alga, are redefined as complex ecosystems considering
the tremendous array of associated microorganisms that satisfy this
concept. The present study focuses on the diversity of the microbiota associated
with the seashore located lichen Rhizocarpon geographicum, recovered
by different culture-dependent methods. Samples harvested from two
sites allowed the isolation and the molecular identification of 68 fungal isolates
distributed in 43 phylogenetic groups, 15 bacterial isolates distributed
in five taxonomic groups and three microalgae belonging to two species.
Moreover, for 12 fungal isolates belonging to 10 different taxa, the genus
was not described in GenBank. These fungal species have never been
sequenced or described and therefore non-studied. All these findings highlight
the novel and high diversity of the microflora associated with R. geographicum.
While many species disappear every day, this work suggests
that coastal and wild environments still contain an unrevealed variety to
offer and that lichens constitute a great reservoir of new microbial taxa
which can be recovered by multiplying the culture-dependent techniques.
- Sunday, 12 March 2023 19:22