Lichens of Mugikusa Pass and the adjacent areas in Kita-Yatsugatake Mountains, central Japan, were surveyed from 2011 to 2018. As the result of taxonomic examinations of 390 out of 442 specimens, 146 taxa (145 species 1 subspecies 1 variety) in 64 genera were recognized. The 141 ITS rDNA sequences were successfully obtained from 84 species. Among them, Menegazzia caviisidia and Usnea longissima are known as the species of “Near Threatened (NT)” category in the Red List 2020 of Ministry of the Environment, Government of Japan; Baeomyces carneus and Cetrelia cetrarioides were insufficiently understood in Japan; and Peltigera neopolydactyla and Rhizocarpon hochstetteri are species requiring further taxonomic examination. These species were discussed in detail in the species list. Keywords: DNA barcoding, inventory, ITS rDNA, lichenized fungi, mycota, subalpine, taxonomy.