Two new species of the lichen genus Oxneriaria are discovered in Pakistan, namely Oxneriaria iqbalii and O. kohistaniensis. Oxneriaria iqbalii is characterized by its non-radiating peripheral areoles, indistinct prothallus, 0.3–0.8 mm apothecial disc, bigger ascospores (20–26×10–14 µm) and conidia (15–30×c. 0.5 µm), whereas Oxneriaria kohistaniensis can be characterized by peripherally non-elongated areoles, indistinct prothallus, simple paraphyses, smaller ascospores (15–20×9–12 µm) and chemistry (cryptostictic acid and 2’-O-methysulphurellin) as lichen substances. Sequences of nrITS and nrLSU region of newly reported species nested as separate taxa in phylogenetic analyses of current study. This is the frst report of the genus Oxneriaria from the country. Keywords: Khyber; Pakhtunkhwa; Phylogeny; Swat; Taxonomy.