Lead (Pb) is used in many industrial applications and is a toxic heavy metal that causes health hazards. Lichens
are symbiotic associations that are environment friendly in removing toxic elements from the polluted environment.
They can accumulate heavy metals and are therefore considered in biomonitoring of metal pollution. In
this study, biochemical markers due to lead toxicity on Cladonia convoluta were revealed using two-dimensional
correlation (2DCOS) spectra analysis. The 2DCOS enhances the resolution of the spectrum and gives more information
on the interaction of functional groups within and between molecules which cannot be revealed from
conventional spectra. Thus, 2DCOS is an efficient tool in monitoring even minute spectral changes in complex
spectra. The results of conventional IR analysis showed changes in the band area. It follows in the order polysaccharides
> proteins > lipids due to lead toxicity. The results of 2D correlation spectra show predominant
changes at the pyranose ring. The hetero asynchronous map show correlation changes of pyranose ring with lipid
and proteins. It reveals a change in carbohydrate synthesis due to toxicity. The highest positive and negative PCA
loading values show deformation pyranose ring to glucan band. The results of HCA show samples are well
separated into two major clusters with high separation values. ROC analysis gives good reliability of the test.
Hence, 2DCOS coupled with chemometric analysis helps in understanding the biomolecular changes in C. convoluta
effectively.
Keywords: 2DCOS; Lichens; Polysaccharides; ROC; Pb.
- Author:
- Karthikeyan S., Gurbanov R. & Unal D.
- Year:
- 2022
- Journal:
- Vibrational Spectroscopy
- Pages:
- 119: 103341 [9 p.]
- Pdf:
- 0
- Id:
- 34125
- Submitter:
- zdenek
- Post_time:
- Wednesday, 02 February 2022 09:26