Five known triterpenes, β-amyrone (1), isoarborinol acetate (2), hopane-6α,22-diol (3), hopane-22-ol (4), hopane-6α,16β,22-triol (5), along with two esgostane-type sterols, 5α,8α-esgosterol peroxide (6), brassicasterol (7) were isolated from the lichen Ramalina peruviana Ach. (Ramalinaceae). Their chemical structures were elucidated by spectroscopic data analysis and comparison with those reported in the literature. Except 2, this is the first time these compounds are reported in Ramalina genus. Keywords: Ramalina peruviana, triterpenes, esgostane-type sterols.