[in Chinese with English abstract:] Agrobacterium tumefaciens‐mediated transformation (ATMT) was successfully applied to the lichen forming fungus Cladonia metacorallifera. Vegetative mycelium of this fungus was used for transformation by ATMT. The genes for hygromycin resistance (hph) and the enhanced green fluorescent protein (EGFP), both under the control of a trpC promoter, were employed as selection markers. PCR amplification of hph and the observation of fluorescence signal by confocal microscopy showed that C. metacorallifera was successfully transformed by ATMT. Key words: Cladonia metacorallifera, Agrobacterium‐mediated transformation, enhanced green fluorescent protein (EGFP), hygromycin resistence.